Напечатать документ Послать нам письмо Сохранить документ Форумы сайта Вернуться к предыдущей
АКАДЕМИЯ ТРИНИТАРИЗМА На главную страницу
Дискуссии - Наука

Yuri D. Ivanov, Andrey F. Kozlov, Rafael A. Galiullin, Vadim Yu. Tatur, Vadim S. Ziborov, Sergey A. Usanov, Tatyana O. Pleshakova
Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution
Yuri D. Ivanov 1
Andrey F. Kozlov 1
Rafael A. Galiullin 1
Oб авторе - Vadim Yu. Tatur 2
Vadim S. Ziborov 3
Sergey A. Usanov 4
Tatyana O. Pleshakova 1


1 Institute of Biomedical Chemistry, Pogodinskaya st., 10, Moscow 119121, Russia

2 Foundation of Perspective Technologies and Novations, Moscow 115682, Russia

3 Joint Institute for High Temperatures of the Russian Academy of Sciences, Moscow 125412, Russia

4 Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk 220141, Belarus

Received: 27 December 2017 / Revised: 29 March 2018 / Accepted: 4 May 2018 / Published: 21 May 2018


Abstract:

It is known that a charge is generated in water and protein solutions upon their motion; this phenomenon is also observed in analytical systems for atomic force microscopy (AFM)-based fishing. At that, the efficiency of protein fishing correlates with the value of charge, generated upon the motion of the analyzed solution. Earlier, we demonstrated that a pulsed electric field can well be used for the enhancement of the efficiency of AFM-based fishing of low-abundant protein. In this paper, we have demonstrated the influence of a pulsed electric field on the stimulation of the electric charge generation in a solution of low-abundant proteins observed in the injector part of an AFM-based fishing system at 23 °C and 38 °C. Taking this effect into account is important for the development of novel highly sensitive flow-based diagnostic systems, as well as for the development of models describing the influence of a pulsed electric field on pathological processes in the body, hemodynamics, and physicochemical properties of solutions.

Keywords:

analytical flow-based systems; flowing protein solution; charge accumulation


1. Introduction

Development of novel approaches for the detection of low-abundant proteins is of high importance for highly sensitive proteomics. The motion of water and protein solutions through pipes in every analytical system is known to have an electrokinetic effect (usually named as the triboelectric effect), which manifests itself through charge generation [1]. We observed the occurrence of this effect in atomic force microscopy (AFM)-based fishing systems for the detection of low-abundant proteins upon flowing of an analyte solution through an injector into a measuring cell of this system [2,3,4,5]. Moreover, in the paper [2], it was reported that the efficiency of protein detection correlates with the amount of charge (in nanoCoulombs, nC). Studying this effect is very important for modeling hemodynamics and mechanisms of functioning of living systems.

The stimulating effect of a pulsed electric field on the efficiency of protein fishing in an AFM-based fishing system was demonstrated earlier with the example of human cytochrome b5 protein [5]. In the present study, we have used the solution of the same protein to determine the influence of pulsed electromagnetic fields on the charging of a protein solution at ultra-low protein concentrations (10−15 M). It is known that membrane cytochrome b5 is a hydrophobic protein, whose charge at pH = 6 is negative [6,7]. Experiments have been carried out at two temperatures: room temperature of 23 °C (which is typical for functioning of analytical systems) and an elevated temperature of 38 °C (which corresponds to the temperature of human body).

It has been demonstrated that the value of the charge generated in a flow of water or protein solution depends on temperature. The stimulating effect was ~40% for both water and protein solution at 23 °С, and for water and protein solution at 38 °С, this effect was ~40% and 75%, respectively.

Data obtained in this work should be taken into account when modeling the functioning of flow-based diagnostic systems employing AFM-based fishing, as well as other flow-based analytical systems: for instance, nanowire biosensors.

Moreover, taking into account the fact that living systems themselves generate electric fields and pulses, whereas external pulsed electric fields, in their turn, also affect living systems and enzymes [8,9,10], the data obtained in the present study can be interesting for use in modeling pathological processes in human body upon the impact of pulsed electric fields: for instance, in the development of hemodynamic models.


Полный текст доступен в формате PDF (4025Кб)

Separations 2018, 5(2), 29; doi:10.3390/separations5020029



Yuri D. Ivanov, Andrey F. Kozlov, Rafael A. Galiullin, Vadim Yu. Tatur, Vadim S. Ziborov, Sergey A. Usanov, Tatyana O. Pleshakova, Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution // «Академия Тринитаризма», М., Эл № 77-6567, публ.24513, 23.05.2018

[Обсуждение на форуме «Публицистика»]

В начало документа

© Академия Тринитаризма
info@trinitas.ru