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Yu.D. Ivanov, K.A. Malsagova, A.A. Izotov, T.O. Pleshakova, V.Yu. Tatur, S.G. Vesnin, N.D. Ivanova, S.A. Usanov, A.I. Archakov
Yu. D. Ivanova
K.A. Malsagovaa
A.A. Izotova
T.O. Pleshakovaa
V.Yu. Taturb
S.G. Vesninc
N.D. Ivanovad
S.A. Usanove
A.I. Archakova
a Institute of Biomedical Chemistry, ul. Pogodinskaya 10, Moscow, 119121 Russia
b Foundation of Perspective Technologies and Novations, Moscow, Russia
c RES LTD, Moscow, Russia
d Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia
e Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus
Abstract
Microwave radiation at 3.4–4.2 GHz frequency of the cytochrome P450 CYP102A1 (BM3) solution was registered during the lauric acid hydroxylation reaction. The microwave radiation generation was shown to occur following the addition of electron donor NADPH to a system containing an enzyme and a substrate. The radiation occurs for the enzyme solutions with enzyme concentrations of 10−8 and 10−9 М. The microwave radiation effect elicited by the aqueous enzyme solution was observed for the first time. The results obtained can be used to elaborate a new approach to enzyme systems research, including studying of the mechanism of interaction of a functioning enzyme system with microenvironment.
Keywords
Cytochrome P450; Monooxygenase system; Microwave radiation
Biochemistry and Biophysics Reports, doi:10.1016/j.bbrep.2015.12.013
Полный текст доступен в формате PDF (967Кб)
Yu.D. Ivanov, K.A. Malsagova, A.A. Izotov, T.O. Pleshakova, V.Yu. Tatur, S.G. Vesnin, N.D. Ivanova, S.A. Usanov, A.I. Archakov, Detection of microwave radiation of cytochrome CYP102A1 solution during the enzyme reaction // «Академия Тринитаризма», М.,
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